Cytotoxic Effects, Phytochemical and GC/MS Analyses of Bosciasenegalensis.L Leave Extracts
boscia senegalensis, brine shrimp larvae, GC/MS analysis, tramadol, D allose
Abstract
Boscia senegalensis leaves which were traditionally used to relief intestinal pain in Sudan were tested for secondary metabolites and cytotoxic effects. The result indicated a moderate presence of alkaloids, highly presence of tannins, weakly presence of flavonoids and steroids and negative results for saponin, phenolic compound and triterpens. Methanolic extract was tested for cytotoxicity against brine shrimp larvae. Remarkable cytotoxicity was revealed with high value equal to 1.975 µg/ml. Also the extract was subjected to separation by column chromatography technique, four fractions were obtained. The fractions tested for cytotoxicity against brine shrimp larvae again. F 1 , F 2 , F 3 and F 4 represented high values equal to 66.13. 11.07, 1.74 and 99.41 µg/ml respectively. F 3 with high cytotoxicity was chosen for gas chromatography / mass spectrometry analysis. Thirty-five compounds were not recorded in any previous work in available literature were obtained. The high cytotoxicity of this fraction due to presence of octadecenoic and n-hexadecenoic acid which are known to have anticancer activity.
Downloads
How to Cite
References
M Adam Sakine, Y Mahmout, J Gbenou, W Agbodjogbe, M Moudachirou (2011) Effete anti hyper glycémiant des extraits de Boscia senegalensis (Pers.) Lam. Ex Poiret et de Colocynthis vulgaris (L.) Schrad. 9, 268-273.
J Anderson, C Goetz, J Mclaughlin, M Suffness (1973) A blind comparison of simple bench‐top bioassays and human tumour cell cytotoxicities as antitumor prescreens. 2(3), 107-111.
J Harborne (1998) Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 302.
Asish Hazra, Padma Chatterjee (2008) A nontoxic antitumour compound from the leaves of Bauhinia scandens characterized as 1-O-alkyl glycerol by gas-liquid chromatography and evaluation of its antitumour property by Brine Shrimp bioassay. 27(1), 39-43.
K Hostettmann (1991) Assays for Bioactivity. 360.
I Indabawa (2009) Detection and toxicological study of Microsystem by Brine shrimp assay from Microcystis aeruginosa isolated from Kano Burrow pits. 21(5), 209-214.
V Isidrov, V Czyzewska, S Bakier (2011) Determination of royal jelly acid in honey. 24, 387-391.
John Fernada (2005) Toxicology of plant. 3, 1.
T Kalinovsky, M Waheed, W Nusrath, V Ivanov, A Niedzwiecki, M Rath (2004) Modulation of N-methyl -N-nitrosoutea induced mammary tumors in Sarague -Dawley rats by combination of lysine, proline, arginine, ascorbic acid and green tea extract.
N Mahamat, M Yaya, M Dijoux-Franca, J Gbenou, M Moudachirou (2012) In vitro antihyperglycaemic effect of glucocapparin isolated from the seeds of Boscia senegalensis (Pers.) Lam. ex Poiret. 11, 6345-6349.
M Mackeen, A Ali, N Lajis, K Kawazu, Z Hassan, M Amran, M Habsah, L Mooi, S Mohamed (2000) Antimicrobial, antioxidant, antitumour-promoting and cytotoxic activities of different plant part extracts of Garcinia atroviridis Griff. ex T. Anders.. 72(3), 395-402.
D Mclaughin, C Change, D Smith (1991) Author Index. 9, 383-401.
Jerry Mclaughlin, Lingling Rogers, Jon Anderson (1998) The Use of Biological Assays to Evaluate Botanicals. 32(2), 513-524.
Jerry Mclaughlin, Ching-Jer Chang, David Smith (1991) Simple Bench-Top Bioassays (Brine Shrimp and Potato Discs) for the Discovery of Plant Antitumor Compounds. 112-137.
Buetusiwa Menavuvu, Wayoon Poonperm, Khim Leang, Naoki Noguchi, Hiromi Okada, Kenji Morimoto, Tom Granström, Goro Takada, Ken Izumori (2006) Efficient biosynthesis of d-allose from d-psicose by cross-linked recombinant l-rhamnose isomerase: Separation of product by ethanol crystallization. 101(4), 340-345.
B Meyer, N Ferrigni, J Putnam, L Jacobsen, D Nichols, J Mclaughlin (1982) Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents. 45(05), 31-34.
M Moshi, J Cosam, . Mbwambo, M Kapingu, M Nkunya (2004) Testing beyond ethno medical claims: Brine shrimp lethality of some Tanzanian plants. 42, 547-551.
R Ngomvougat, H Foyet, R Garabed, R Ziebe (2015) Antioxidant activity and phytochemical constituents of two plants used to manage foot and mouth disease in the Far North Region of Cameroon. 4, 40-46.
Vaughn Nossaman, Usha Ramadhyani, Philip Kadowitz, Bobby Nossaman (2010) Advances in Perioperative Pain Management: Use of Medications with Dual Analgesic Mechanisms, Tramadol & Tapentadol. 28(4), 647-666.
Edward Hanna (2008) Radiative forcing of climate change: expanding the concept and addressing uncertainties. By the National Research Council (NRC). National Academies Press, Washington DC, USA, 2005. 207 pp. Paperback. 62(4), 109-109.
C Orwa, A Mutua, R Kindt, R Jamnadass, S Anthony (2009) Agroforestree Database: a tree reference and selection guide version 4.
R Padmaja, P Arun, D Prashanth, M Deepak, A Amit, M Anjana (2002) Brine shrimp lethality bioassay of selected Indian medicinal plants. 73(6), 508-510.
M Sakine, Y Mahmout, M Dijoux-Franca, J Gbenou, M Moudachirou (2012) In vitro antihyperglycaemic effect of glucocapparin isolated from the seeds of Boscia senegalensis (Pers.) Lam. ex Poiret. 11(23), 6345-6349.
A Samy, Hussein, A Samir, Abdelaal, K Hany, Ismail (2017) Effect of tramadol drug on some biochemical and immunological parameters in albino male rats; evaluation of possible reversal following its withdrawal. 33(2), 418-429.
Pablo Solis, Colin Wright, Margaret Anderson, Mahabir Gupta, J Phillipson (1993) A Microwell Cytotoxicity Assay usingArtemia salina(Brine Shrimp). 59(03), 250-252.
Li Sui, Youyi Dong, Yasuo Watanabe, Fuminori Yamaguchi, Naoya Hatano, Ikuko Tsukamoto, Ken Izumori, Masaaki Tokuda (2005) The inhibitory effect and possible mechanisms of D-allose on cancer cell proliferation. 25, 2639-2644.
Published
2021-03-08
Issue
Section
License
Copyright (c) 2021 Authors and Global Journals Private Limited
This work is licensed under a Creative Commons Attribution 4.0 International License.