DNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers

Chiedozie Joel Uka; Daniel Awomukwu; Bio Louis Nyananyo; Chiedozie Joel Uka

Authors

Chiedozie Joel Uka
Daniel Awomukwu
Bio Louis Nyananyo
Chiedozie Joel Uka

Phyllanthus species, DNA extraction, PCR amplification, nucleotide sequences, BLAST, Bold Systems, rbcL, matk, Barcoding

Abstract

Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were extracted, purified, PCR amplified and sequenced using plastid Ribulose -1,5 bisphosphate carboxylase large chain (rbcL a) and Maturase K (matK) genetic marker to identify unknown Phyllanthus species. The plastid region revealed that the Phyllanthus species were able to be amplified optimally for sequencing. The results of the nucleotide sequences were further compared on Basic Local Alignment Sequence Tool (BLAST) on Gen Bank and Bold Systems for validation. Results revealed that the closely related species, P. niruroides Mull_Arg. and P. odontadenius Mull-Arg. had no DNA record to separate them on both GenBank and Bold Systems using both rbcL and matK gene regions while P. amarus Schum and Thonn and P. urinaria Linn. were clearly compatible with other works. P. muellerianus (O. Ktze) Excel was only compatible with other works using matK gene region but none for rbcL.

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DNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers. (2015). Global Journal of Science Frontier Research, 15(G1), 1-12. https://www.journalofscience.org/index.php/GJSFR/article/view/1658

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