The T-RFLP Analysis of Methanogenic Community during the an Aerobic Fermentation of Tofu Liquid Waste
methanogenic, euryarchaeota, methanosphaerula palustris, terminal restriction fragment length polymorphism (TRFLP), tofu liquid waste
Abstract
The increase energy demand by the due to the growth of human population could cause decreasing fossil fuel energy. In near future energy demand will increase rapidly in the future. All those problems require each country to produce and to use renewal energy. Biogas is one of the renewable energy sources that can be used as the alternative energy. This is a kind of methane gas which is formed of various organic waste biomasses such as tofu waste. The aim of this research was to study the dynamic structure of methanogens community in the anaerobic digestion process by T-RFLP method. Sample was taken from anaerobic biodigester on day: 0, 5, 10, 15, and 20 to measure the pH, temperature, CH4, and molecular analysis. The dynamic of methanogens community during anaerobic digestion was observed by the TRFLP (Terminal Restriction Fragment Length Polymorphism) technique. The restriction enzyme used in that method was Alu1. The Shannon-Wiener (H') index was used to analyze the community diversity.
Downloads
How to Cite
References
Q Cadillo, J Yavitt, S Zinder (2009) Methanosphaerula palustris gen. Nov.,sp.nov.,a Hydrogenotrophic Methanogen Isolated from a Minerotrophic Fen Peatland. 59(Pt 5), 928-935.
T De Mez, A Stams, J Reith, G Zeeman (2003) Methane Production by Anaerobic Digestion of Wastewater and Solid Wastes.
S Dubey (2005) MICROBIAL ECOLOGY OF ME THANE EMISSION IN RICE AGROECOSYSTEM: A REVIEW. 3(2), 1-27.
Thomas Freitag, James Prosser (2009) Correlation of Methane Production and Functional Gene Transcriptional Activity in a Peat Soil. 75(21), 6679-6687.
P Galand, H Fritze, K Yrjala (2003) Microsite-dependent changes in methanogenic populations in a boreal oligotrophic fen. 5, 1133-1143.
O Kotsyurbenko, R Chin, K, J Glagolev, M, V Stubner, S Simankova, M, V Nozhevnikova, A, N, Conrad (2004) Acetoclastic and Hydrogenotrophic Methane Production and methanogenic Populations in an Acidic West-Siberian Peat Bog. 6, 1159-1173.
C Krebs (1972) Ecology: The Experimental Analysis of Distribution and Abundance.
D Liu, W Ding, Z Jia, Z Cai (2011) Relation between methanogenic archaea and methane production potential in selected natural wetland ecosystems across China. 8(2), 329-338.
T Lukow, Peter Thomas, Werner Dun¢eld, Liesack (2000) Use of the T-RFLP technique to assess spatial and temporal changes in the bacterial community structure within an agricultural soil planted with transgenic and non-transgenic potato plants. 32(3), 241-247.
Terence Marsh, Paul Saxman, James Cole, James Tiedje (2000) Terminal Restriction Fragment Length Polymorphism Analysis Program, a Web-Based Research Tool for Microbial Community Analysis. 66(8), 3616-3620.
M Metje, P Frenzel (2005) Effect of Temperature on Anaerobic Ethanol Oxidation and Methanogenesis in Acidic Peat from a Northern Wetland. 71, 8191-8200.
A Pangastuti (2008) Analisis Komunitas Bakteri Selama Tahapan Perkembangan Larva.
L Raskin, R Mackie, K Mcmahon, M Griffin (2007) Methanogenic Population Dynamics during Start-Up of Anaerobic Digesters Treating Municipal Solid Waste and BiosolidsMatt. 5, 342-355.
Reinoud Segers (1998) Methane production and methane consumption: a review of processes underlying wetland methane fluxes. 41(1), 23-51.
Suryandono Dan Wagiman (2004) Laju produksi Biogas dari Limbah Cair Tahu.
E Stoica (2009) Application of T-RFLP analysis to the study of the coastal Black Sea bacterioplankton. 14(5), 4710-4719.
B Svensson (1984) Different temperature optima for methane formation when enrichments from acid peat are supplemented with acetate or hydrogen. 48, 389-394.
Rudolf Thauer (1998) Biochemistry of methanogenesis: a tribute to Marjory Stephenson:1998 Marjory Stephenson Prize Lecture. 144(9), 2377-2406.
L Devetter, E Beaver, J Colquhoun, J Zalapa, R Harbut (2009) Comparison of nonstructural carbohydrates across cranberry cultivars. 81(6), 321-326.
Published
2014-09-06
Issue
Section
License
Copyright (c) 2014 Authors and Global Journals Private Limited
This work is licensed under a Creative Commons Attribution 4.0 International License.