NET-Seq and MNase-seq Analysis of Gene Response of S.Cerevisiae in Galactose Environment

Abstract

Introduction-Earth has diverse and extreme environments so, for an organism to survive, it is vital that it is able to respond to changes in temperature, availability of food and even other organisms. For organisms to function properly, and adapt to these conditions, gene expression at the right time is essential. Gene expression is the process by which information in DNA is transferred to RNA molecules that create proteins for various functions. The control over the creation of proteins for a situation is essentially what gene expression and gene regulation is1. For instance Himalayan rabbits that were bred at temperatures less than 20 C had black sections of fur, while those bred at 35 C or more had completely white fur, suggesting differentiated gene expression for a changed condition-temperature2. The first step of gene expression is transcription, therefore measuring the levels of transcription of genes in different conditions can be an indicator of the effects of gene regulation. In order for transcription to occur, mRNA segments, named "transcripts", are copied from DNA, so finding which mRNA transcripts are copied can indicate gene expression for various times and conditions. SAGE and Northern Blot techniques measure mRNA segments to analyze gene expression..