Study on Enzyme Activity in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural Byproducts
α-amylase, bacillus lichenoformis, low cost medium, agricultural by products, fermentation, alkaline enzyme
Abstract
Production of α-amylase enzyme by Bacillus Lichenoformis using stirred tank fermentor (BIOSTAT -E) was carried out. The strain was obtained from National Chemical Laboratory, Pune, India. Corn starch is used as the substrate. The enzyme production was studied by changing the various parameters like temperature, pH, rpm and substrate concentration. The enzyme activity shows maximum at a temperature of 350C -370C, pH 8 and 300 rpm. The maximum enzyme production was achieved, for 1% concentration of cornstarch at 35.60C and pH 9 using the fermentation medium contains yeast extract and peptone and the enzyme activity was found to be 55.93 DUN/ml.
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2014-12-25
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